Spectral Compensation for Linear-Logarithmic Flow Cytometry Acquisitions
نویسندگان
چکیده
منابع مشابه
Spectral compensation for flow cytometry: visualization artifacts, limitations, and caveats.
BACKGROUND In multicolor flow cytometric analysis, compensation for spectral overlap is nearly always necessary. For the most part, such compensation has been relatively simple, producing the desired rectilinear distributions. However, in the realm of multicolor analysis, visualization of compensated often results in unexpected distributions, principally the appearance of a large number of even...
متن کاملSpectral Flow Cytometry
Figure 1: Schematic of the particles in the fluid flow and interaction region of the laser within the flow cell. A key and continuing goal in the development of flow cytometry techniques is the ability to measure ever more parameters for each particle under test. Work carried out by Prof John Nolan’s group at La Jolla Bioengineering Institute, and reported recently by Watson et. al.[1] in Cytom...
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Propidium Iodide is a fluorochrome that is used to measure the DNA content of individual cells, taken from solid tissues, with a flow cytometer. Compensation for spectral cross-over of this fluorochrome still leads to compensation results that are depending on operator experience. We present a data-driven compensation (DDC) algorithm that is designed to automatically compensate combined DNA phe...
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BACKGROUND While conventional multiparameter flow cytometers have proven highly successful, there are several types of analytical measurements that would benefit from a more comprehensive and flexible approach to spectral analysis including, but certainly not limited to spectral deconvolution of overlapping emission spectra, fluorescence resonance energy transfer measurements, metachromic dye a...
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Lasers are the principle light sources of flow cytometers. Most modern benchtop instruments feature air-cooled argon-ion laser emitting 10-25 mW of light at 488 nm. This unit covers the various types of lasers available and the qualities that make them suitable or unsuitable for use in flow cytometers. Also included is a discussion of future directions, particularly in the development of solid-...
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ژورنال
عنوان ژورنال: Journal of Biomedical Science and Engineering
سال: 2014
ISSN: 1937-6871,1937-688X
DOI: 10.4236/jbise.2014.79063